The migration of epidermal keratinocytes is a fundamental element of cutaneous wound healing in which diverse members of the microRNA (miRNA) family have now been implicated. Recently, we observed that miR-184 expression was induced following high-density scratch wounding of keratinocyte monolayers, suggesting a role for miR-184 in keratinocyte migration. We therefore transfected keratinocytes with a miR-184 mimic or a miR-184 inhibitor and evaluated the movement of the cells by time-lapse microscopy in order to test the hypothesis that miR-184 regulates the ability of keratinocytes to migrate during wound healing.  The time-lapse microscopy data of epidermal keratinocytes loaded with miR-184 mimic, a miR-184 inhibitor or their respective control oligonucleotides are presented. Cells were transfected with 100 nM of the indicated oligonucleotide using nucleofection and allowed to approach confluence in a 6-well plate. A single scratch was then made across the cell monolayers with a pipette tip and cells monitored at 30-minute intervals by time-lapse microscopy, for up to 72 hours. These data files will enable comparisons to be made with other time-lapse studies of keratinocyte migration in the context of scratch assays. The data may also prove useful for the development and validation of mathematical and computational models of miRNA-mediated migration.