100 14 369 3 1068 document 369 Migration of keratinocytes loaded with miR-184 inhibitor.mp4 video/mp4 9ceaa6ca0e0470ca20dece38553c5bf2 MD5 3807557 2021-08-26 18:25:40 https://opendata.ljmu.ac.uk/id/eprint/100/1/Migration%20of%20keratinocytes%20loaded%20with%20miR-184%20inhibitor.mp4 100 1 1 video/mp4 video/mp4 en public cc_by_nd_4

Migration of keratinocytes loaded with miR-184 inhibitor.mp4

data Audiovisual 370 3 1070 document 370 Migration of keratinocytes loaded with non-targeting mimic control.mp4 video/mp4 dbc5204a6dadc98abc871bebcecce12e MD5 5797659 2021-08-26 18:25:43 https://opendata.ljmu.ac.uk/id/eprint/100/2/Migration%20of%20keratinocytes%20loaded%20with%20non-targeting%20mimic%20control.mp4 100 2 2 video/mp4 video/mp4 en public cc_by_nd_4

Migration of keratinocytes loaded with non-targeting mimic control.mp4

data Audiovisual 371 3 1072 document 371 Migration of keratinocytes loaded with non-targeting inhibitor control.mp4 video/mp4 7d9340bb7ce58ce00a7c73aeaf6fd3df MD5 6794464 2021-08-26 18:25:46 https://opendata.ljmu.ac.uk/id/eprint/100/3/Migration%20of%20keratinocytes%20loaded%20with%20non-targeting%20inhibitor%20control.mp4 100 3 3 video/mp4 video/mp4 en public cc_by_nd_4

Migration of keratinocytes loaded with non-targeting inhibitor control.mp4

data Audiovisual 372 3 1074 document 372 Migration of keratinocytes loaded with miR-184 mimic.mp4 video/mp4 aaf7ca4dc00fbd7fd78e8835f52c9fcc MD5 8371968 2021-08-26 18:25:51 https://opendata.ljmu.ac.uk/id/eprint/100/4/Migration%20of%20keratinocytes%20loaded%20with%20miR-184%20mimic.mp4 100 4 4 video/mp4 video/mp4 en public cc_by_nd_4

Migration of keratinocytes loaded with miR-184 mimic.mp4

data Audiovisual archive 7 disk0/00/00/01/00 2021-08-27 09:19:39 2024-08-22 10:17:12 2021-08-27 09:19:39 data_collection show 7 Richardson Adam Owens Daniel 0000-0002-1908-8677 Ross Kehinde 0000-0003-0252-1152 datapub pharmacy public miR-184; MicroRNA; Skin; Epidermis; Keratinocyte; Migration; Wound Healing. The migration of epidermal keratinocytes is a fundamental element of cutaneous wound healing in which diverse members of the microRNA (miRNA) family have now been implicated. Recently, we observed that miR-184 expression was induced following high-density scratch wounding of keratinocyte monolayers, suggesting a role for miR-184 in keratinocyte migration. We therefore transfected keratinocytes with a miR-184 mimic or a miR-184 inhibitor and evaluated the movement of the cells by time-lapse microscopy in order to test the hypothesis that miR-184 regulates the ability of keratinocytes to migrate during wound healing. The time-lapse microscopy data of epidermal keratinocytes loaded with miR-184 mimic, a miR-184 inhibitor or their respective control oligonucleotides are presented. Cells were transfected with 100 nM of the indicated oligonucleotide using nucleofection and allowed to approach confluence in a 6-well plate. A single scratch was then made across the cell monolayers with a pipette tip and cells monitored at 30-minute intervals by time-lapse microscopy, for up to 72 hours. These data files will enable comparisons to be made with other time-lapse studies of keratinocyte migration in the context of scratch assays. The data may also prove useful for the development and validation of mathematical and computational models of miRNA-mediated migration. 2021-08-27 published Liverpool John Moores University 10.24377/LJMU.d.00000100 British Skin Foundation Dr Adam Richardson Dr Kehinde Ross British Skin Foundation 7006s http://dx.doi.org/10.13039/501100000296 Confocal Microscopy 7006s MP4 files English 2018-05-21 2018-05-21 https://onlinelibrary.wiley.com/doi/10.1111/bjd.17576 pap 2021-08-27