MicroRNA-184 and its lncRNA sponge uca1 are induced in wounded keratinocytes in a store-operated calcium entry-dependent manner.

Richardson, Adam, Owens, Daniel ORCID: https://orcid.org/0000-0002-1908-8677 and Ross, Kehinde ORCID: https://orcid.org/0000-0003-0252-1152 (2021) MicroRNA-184 and its lncRNA sponge uca1 are induced in wounded keratinocytes in a store-operated calcium entry-dependent manner. [Data Collection]

Abstract

The migration of epidermal keratinocytes is a fundamental element of cutaneous wound healing in which diverse members of the microRNA (miRNA) family have now been implicated. Recently, we observed that miR-184 expression was induced following high-density scratch wounding of keratinocyte monolayers, suggesting a role for miR-184 in keratinocyte migration. We therefore transfected keratinocytes with a miR-184 mimic or a miR-184 inhibitor and evaluated the movement of the cells by time-lapse microscopy in order to test the hypothesis that miR-184 regulates the ability of keratinocytes to migrate during wound healing. The time-lapse microscopy data of epidermal keratinocytes loaded with miR-184 mimic, a miR-184 inhibitor or their respective control oligonucleotides are presented. Cells were transfected with 100 nM of the indicated oligonucleotide using nucleofection and allowed to approach confluence in a 6-well plate. A single scratch was then made across the cell monolayers with a pipette tip and cells monitored at 30-minute intervals by time-lapse microscopy, for up to 72 hours. These data files will enable comparisons to be made with other time-lapse studies of keratinocyte migration in the context of scratch assays. The data may also prove useful for the development and validation of mathematical and computational models of miRNA-mediated migration.

Creators:	Richardson, Adam, Owens, Daniel and Ross, Kehinde
ORCID:	UNSPECIFIEDhttps://orcid.org/0000-0002-1908-8677https://orcid.org/0000-0003-0252-1152
Uncontrolled Keywords:	miR-184; MicroRNA; Skin; Epidermis; Keratinocyte; Migration; Wound Healing.
DOI:	https://doi.org/10.24377/LJMU.d.00000100
Division:	Pharmacy & Biomolecular Sciences
Date Deposited:	27 Aug 2021 09:19
Last Modified:	22 Aug 2024 10:17
Related resources:	Published paper
URI:	https://opendata.ljmu.ac.uk/id/eprint/100
Data collection method:	Confocal Microscopy
Grant number:	7006s
Resource language:	MP4 files
Metadata language:	English
Collection period:	From To 21 May 2018 21 May 2018