LJMU Data Repository

Search LJMU Data Repository

Browse Repository

MicroRNA-184 and its lncRNA sponge uca1 are induced in wounded keratinocytes in a store-operated calcium entry-dependent manner.

Richardson, Adam and Owens, Daniel and Ross, Kehinde (2021) MicroRNA-184 and its lncRNA sponge uca1 are induced in wounded keratinocytes in a store-operated calcium entry-dependent manner. [Data Collection]

Abstract

The migration of epidermal keratinocytes is a fundamental element of cutaneous wound healing in which diverse members of the microRNA (miRNA) family have now been implicated. Recently, we observed that miR-184 expression was induced following high-density scratch wounding of keratinocyte monolayers, suggesting a role for miR-184 in keratinocyte migration. We therefore transfected keratinocytes with a miR-184 mimic or a miR-184 inhibitor and evaluated the movement of the cells by time-lapse microscopy in order to test the hypothesis that miR-184 regulates the ability of keratinocytes to migrate during wound healing. The time-lapse microscopy data of epidermal keratinocytes loaded with miR-184 mimic, a miR-184 inhibitor or their respective control oligonucleotides are presented. Cells were transfected with 100 nM of the indicated oligonucleotide using nucleofection and allowed to approach confluence in a 6-well plate. A single scratch was then made across the cell monolayers with a pipette tip and cells monitored at 30-minute intervals by time-lapse microscopy, for up to 72 hours. These data files will enable comparisons to be made with other time-lapse studies of keratinocyte migration in the context of scratch assays. The data may also prove useful for the development and validation of mathematical and computational models of miRNA-mediated migration.

Creators: Richardson, Adam and Owens, Daniel and Ross, Kehinde
Uncontrolled Keywords: miR-184; MicroRNA; Skin; Epidermis; Keratinocyte; Migration; Wound Healing.
DOI: https://doi.org/10.24377/LJMU.d.00000100
Division: Pharmacy & Biomolecular Sciences
Date Deposited: 27 Aug 2021 09:19
Last Modified: 27 Aug 2021 09:21
Related resources:
URI: https://opendata.ljmu.ac.uk/id/eprint/100

Files

Data

Related resources

Statistics

Actions (Log-in required)

View Item View Item